Lactation stage-dependent protective effects of human milk exosomes against intestinal ischemia-reperfusion injury via lactoferrin/lysozyme-mediated Nrf2/HO-1 and NF-κB pathways

Figure 8 Lactoferrin and lysozyme abundance correlates with pathway activity and barrier protection. A: Levels of tumor necrosis factor-α and interleukin-1β in cell supernatants; B: Western blot analysis of zonula occludens-1 and occludin; C and D: Pearson correlation analysis between lactoferrin/Lysozyme protein abundance and nuclear factor erythroid 2-related factor 2 expression; E and F: Pearson correlation analysis between lactoferrin/Lysozyme protein abundance and p-p65/t-p65 ratio. ^cP < 0.001. TNF-α: Tumor necrosis factor-α; IL-1β: Interleukin-1β; H/R: Hypoxia/reoxygenation; Nrf2: Nuclear factor erythroid 2-related factor 2; ZO-1: Zonula occludens-1; LTF: Lactoferrin; LYZ: Lysozyme.