Cladosporium cladosporioides ameliorates visceral hypersensitivity in irritable bowel syndrome-like mice by inhibiting dectin-1 signaling and mast cell activation

Figure 3 Gavage with Cladosporium cladosporioides downregulated dectin-1/nuclear factor kappa B signaling. A: Reverse transcription-quantitative polymerase chain reaction of dectin-1/spleen tyrosine kinase/caspase recruitment domain family member 9 mRNA expression among groups. One outlier identified in saline + vehicle group in the mRNA expression analysis of caspase recruitment domain family member 9 and removed with Turkey outlier analysis; B: Western blot analysis of dectin-1/p65 protein expression; C: Quantification of dectin-1 protein levels normalized to β-actin levels. Data are presented as the mean ± SD. Two-way ANOVA (n = 7). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. TNBS: Trinitrobenzene sulfonic acid; Syk: Spleen tyrosine kinase; Card9: Caspase recruitment domain family member 9; NF-κB: Nuclear factor kappa B.