Extracts of Gleditsia sinensis Lam. thorn ameliorates ulcerative colitis through PPARγ/GPX4 pathway-mediated inhibition of enterocyte ferroptosis

Figure 4 Extracts from Gleditsia sinensis thorns reduces lipid peroxidation by inhibiting the generation of nitric oxide and reactive oxygen species. A: Detection of nitric oxide levels in Caco-2 cells by flow cytometry; B: Detection of reactive oxygen species levels in Caco-2 cells by flow cytometry. Data are presented as mean ± SD (n = 3). ^aP < 0.05, ^cP < 0.001, and ^dP < 0.0001. DSS: Dextran sulfate sodium; EGST: Extracts from Gleditsia sinensis thorns; NO: Nitric oxide; ROS: Reactive oxygen species.