P-element-induced wimpy testis-interacting RNA: A biomarker for gastric cancer diagnosis, progression, and prognosis

Figure 1 P-element-induced wimpy testis-interacting RNA synthesis mechanism. A: Within the cell nucleus, double-stranded P-element-induced wimpy testis (PIWI)-interacting RNA clusters undergo transcription processes to generate PIWI-interacting RNA (PiRNA) precursors, which can further participate in post-transcriptional silencing regulation; B: In mitochondria-associated processes, Zucchini endoribonuclease protein facilitates the processing of PiRNA precursors into intermediate PiRNA molecules. These intermediates form PiRNA/PIWI complexes with PIWI protein, enabling protein interactions in the nucleus or cytoplasm and post-transcriptional silencing; C: Transposon-related sequences contribute to the generation of PiRNA precursors, which subsequently enter subsequent pathways such as the ping-pong mechanism; D: In the ping-pong mechanism, Aubergine (AUB) protein binds to PiRNA to form AUB-PiRNA complexes; E: These AUB-PiRNA complexes interact with Argonaute 3 protein and other factors, achieving target transposon sequence silencing and PiRNA sequence amplification through the ping-pong cycle, thereby maintaining PiRNA biosynthesis and functional regulation; F: AUB-piRNA complexes participate in the ping-pong cycle with AGO3 to cleave transposon transcripts, amplifying piRNA pools and reinforcing transposon silencing. AGO3: Argonaute 3; Aub: Aubergine; piRISC: P-element-induced wimpy testis-interacting RNA-induced silencing complex; PiRNA: P-element-induced wimpy testis-interacting RNA; PIWI: P-element-induced wimpy testis; Zuc: Zucchini endoribonuclease.