Helicobacter pylori promotes gastric cancer progression by upregulating EPS8L3 to inhibit ferroptosis

Figure 3 Overexpression of epidermal growth factor receptor kinase substrate 8-like protein 3 enhances proliferation, migration, and invasion of gastric cancer cells in vitro. A: Western blot analysis confirmed the efficiency of epidermal growth factor receptor kinase substrate 8-like protein 3 (EPS8L3) overexpression; B and C: Colony formation assays showed enhanced colony formation in gastric cancer cells upon EPS8L3 overexpression; D and E: Overexpression of EPS8L3 significantly increased cell proliferation in MGC-803 and HGC-27 cells; F: Invasion and migration capacities were markedly enhanced in MGC-803 and HGC-27 cells upon EPS8L3 overexpression; G and H: Cell scratch assay revealed that EPS8L3 overexpression significantly increased migration in MGC-803 cells. Data are shown as the mean ± SD (n = 3). ^aP < 0.05 vs control, ^bP < 0.01 vs control, and ^cP < 0.001 vs control. EPS8L3: Epidermal growth factor receptor kinase substrate 8-like protein 3; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; OE: Overexpression; NC: Negative control.