H3K18 La-METTL5-CCT2 signaling axis promotes colorectal cancer progression by inhibiting pyroptosis

Figure 4 H3K18 La-METTL5-CCT2 signaling axis promotes tumor progression by inhibiting pyroptosis in colorectal cancer cells. A and B: Colorectal cancer samples were divided into high- and low-expression groups based on CCT2 expression levels, and differentially expressed genes between the two groups were identified. KEGG pathway enrichment analysis (A). Gene set enrichment analysis for the pyroptosis signaling pathway (B). Bubbles size represents GeneRatio, and bubble color indicates -log10(P value); C: Gene set enrichment analysis of transcriptome sequencing data of METTL5-knockdown SW480 cells for pyroptosis-related genes; D: SW480 cells in the non-targeted short hairpin RNA control (sh-NC) and METTL5-knockdown (sh-METTL5) groups were treated with medium containing phosphate-buffered saline or 5 mmol/L sodium lactate for 24 hours. Pyroptosis was assessed by flow cytometry. Propidium iodide staining was used to indicate cell membrane permeability, and Caspase-1 activity was used to reflect the activation status of inflammatory caspases; E: SW480 cells in the sh-NC and sh-METTL5 groups were stably transfected with oe-NC or oe-CCT2. Pyroptosis was assessed via flow cytometry and quantified for comparison. PBS: Phosphate-buffered saline; Nala: Sodium lactate. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, and ^dP < 0.0001.