Targeting Jun N-terminal kinase phosphorylation: A human-derived hepatoprotective peptide human liver transplantation peptide 1 attenuates hepatic ischemia-reperfusion injury

Figure 2 Screening for polypeptides with potential hepatic ischemia-reperfusion injury-alleviating effects. A: Chemical structure of ‘AVPEGFVIPRGNVL’; B: Conservativeness analysis of the ‘AVPEGFVIPRGNVL’ sequence; C: Mass spectrometry identification of the purity of ‘AVPEGFVIPRGNVL’; D: Mass spectrometry analysis of ‘AVPEGFVIPRGNVL’; E: Representative confocal microscopy images of AML12 cells under normal conditions. Cells were stained with the synthetic human liver transplantation peptide 1-fluorescein isothiocyanate peptide (green) and the nuclear dye DAPI (blue), scale bar = 10 μm. HLTP1: Human liver transplantation peptide 1; FITC: Fluorescein isothiocyanate; RT: Retention time.