γδ T cells exacerbate intestinal fibrosis in mice with experimental colitis via activation of the CD73-adenosine receptor-cyclic AMP signaling pathway

Figure 3 CD73 was downregulated after γδ T cells depletion in mice with intestinal fibrosis. A and B: Immunohistochemistry (IHC) staining of CD73 and the quantification of average optical density (OD) of CD73 in the colon tissues. Quantification of IHC staining expressed as mean OD. Scale bars: 50 μm; C and D: Western blot and the quantification result of the expression levels of interleukin (IL)-17A, transforming growth factor-beta, interferon γ and IL10 using colon tissues in each group (n = 3); E and F: IHC staining of IL-17A, transforming growth factor-beta, interferon γ and IL10 and the quantification of average OD of these proteins in the colon tissues. Quantification of IHC staining expressed as mean OD. Scale bars: 50 μm. Data were presented as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. TNBS: 2,4,6-trinitrobenzenesulfonic acid; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon.