γδ T cells exacerbate intestinal fibrosis in mice with experimental colitis via activation of the CD73-adenosine receptor-cyclic AMP signaling pathway

Figure 2 Reinfusion of γδ T cells aggravated intestinal fibrosis. A: Experimental scheme of 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced intestinal fibrosis model. γδ T cells were depleted using GL3 antibody before TNBS administration. For reinfusion experiment, γδ T cells were reinfused via intraperitoneally injection after TNBS administration; B: TCR staining of the colon tissues following TNBS treatment in each group at week 14. Scale bars: 50 μm; C: Body weight changes in the mice in each group during the experimental period (n = 5); D: Disease activity index score evaluated in the intestinal fibrosis treated with GL3 antibody administration or γδ T cells reinfusion (n = 5); E: Colon morphologies in each group at the end of week 14; F: The representative image for hematoxylin and eosin and Masson staining of colon in each group. Orange arrow indicated areas of immune cell infiltration. Scale bars: 100 μm. Data were presented as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. TNBS: 2,4,6-trinitrobenzenesulfonic acid; DAI: Disease activity index; H&E: Hematoxylin and eosin.