Gastric cancer-derived exosomal indoleamine 2,3 dioxygenase 1 drives immunosuppression in gastric cancer by suppressing dendritic cell maturation

Figure 4 Gastric cancer-derived exosomal indoleamine 2,3 dioxygenase 1 promotes tumor growth in mice. A subcutaneous tumor mouse model was established and treated with unmodified exosomes, indoleamine 2,3 dioxygenase 1-silenced exosomes, and negative control exosomes, respectively (n = 8 group). A: The excised tumors in four group were imaged; B: Tumor volumes were measured every 7 days; C: The weights of excised tumors were measured; D-G: Ki67 and indoleamine 2,3 dioxygenase 1 protein levels were examined using immunohistochemistry. Data were presented as mean ± SD. ^aP < 0.01. exo: Exosomal; IDO1: Indoleamine 2,3 dioxygenase 1; IHC: Immunohistochemistry; PBS: Phosphate buffered saline; sh-IDO1: Short hairpin RNA targeting indoleamine 2,3 dioxygenase 1; sh-NC: Short hairpin RNA targeting negative control.