Impairment of the intestinal epithelium barrier through alteration of proliferation and differentiation balance in alcohol use disorder

Figure 5 Wnt target gene Axin2 is not implicated in hyperproliferation in alcohol use disorder. A: Multiplex immunofluorescence staining of duodenal biopsies showing Axin2 (green), a Wnt target gene; Ki67 (red) for proliferating cells; OLFM4 (yellow) for stem and stem-like cells; and DAPI (blue) for nuclei; B: Quantification of Axin2 fluorescence intensity, normalized to the total crypt area and expressed as a percentage showing a non-significant trend towards decreased Axin2 fluorescence intensity in particularly in alcohol use disorder (AUD)-altered patients. Data are presented as mean ± SD. Scale bar (200 μm). Healthy (n = 5); AUD normal (n = 6); AUD altered (n = 5). AUD: Alcohol use disorder.