Hua-Zhuo-Jie-Du formula alleviates colitis-associated colorectal cancer via regulating phosphatidylinositol 3-kinase/protein kinase B signaling pathway

Figure 4 Hua-Zhuo-Jie-Du formula inhibited tumor proliferation and the phosphatidylinositol 3-kinase/protein kinase B pathway in colitis-associated colorectal cancer mice. A: Representative images of Ki67 staining; B: Quantitative analysis of Ki67-positive cell staining; C: Detection of the proteins associated with the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway by Western blot analysis; β-actin served as a loading control; D: Quantitative analysis of the expression levels of phosphorylated-PI3K/PI3K; E: Quantitative analysis of the expression levels of phosphorylated-AKT/AKT. Data are shown as the mean ± SD. ^bP < 0.01 vs control group. ^cP < 0.05 vs model group. ^dP < 0.01 vs model group. HZJDF-L: Low-dose Hua-Zhuo-Jie-Du formula; HZJDF-M: Medium-dose Hua-Zhuo-Jie-Du formula; HZJDF-H: High-dose Hua-Zhuo-Jie-Du formula; PI3K: Phosphatidylinositol 3-kinase; p-PI3K: Phosphorylated-phosphatidylinositol 3-kinase; AKT: Protein kinase B; p-AKT: Phosphorylated-protein kinase B.