Gram-negative bacteria and endotoxins promote hepatocellular carcinoma progression through the recruitment of myeloid-derived suppressor cells

Figure 3 Gram-negative bacteria and endotoxins affected the survival of hepatocellular carcinoma patients through recruitment of monocytic myeloid-derived suppressor cells. A: Relative expression of lipopolysaccharide (LPS) in LPS^low and LPS^high groups; B: Kaplan-Meier survival analysis of LPS^low and LPS^high groups; C: EUB338 fluorescence in situ hybridization of LPS^low and LPS^high groups (LPS^low, n = 47; LPS^high, n = 46); D and E: The relative expression levels of CD33 and CD8 in LPS^low and LPS^high groups (LPS^low, n = 16; LPS^high, n = 14); F: Representative images of IF staining with CD33⁺CD14⁺ cells in group of LPS^low and LPS^high (LPS^low, n = 16; LPS^high, n = 14); G: The flow cytometry data of MDSCs in LPS^low and LPS^high groups (LPS^low, n = 8, LPS^high, n = 6). ^aP < 0.05, ^bP < 0.01; ^cP < 0.0001. LPS: Lipopolysaccharide.