Kinetochore-associated protein 1 as a prognostic biomarker in gastric cancer: Integrative bioinformatics and experimental validation

Figure 9 Kinetochore-associated protein 1 knockdown inhibited gastric cancer development in vitro and in vivo. A and B: Kinetochore-associated protein 1 expression of gastric cancer cells and the knockdown efficiency of short hairpin RNA (shRNA) were detected; C-I: The colony formation, wound-healing, and Transwell assays were used in both control and sh-kinetochore-associated protein 1 NUGC3 groups, with the corresponding statistical analyses presented; J: Expression of cyclin-dependent kinase 4, cyclin-dependent kinase 6 and cyclin D1 was analyzed by western blotting; K: The scheme of xenograft tumor and corresponding tumor volume was measured; L and M: Tumor images were captured, and tumor weights were measured post-sacrifice in the mice. Data are presented as mean ± SD (n = 3 or 6, depending on the experiment). KNTC1: Kinetochore-associated protein 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; CDK: Cyclin-dependent kinase.