Kinetochore-associated protein 1 as a prognostic biomarker in gastric cancer: Integrative bioinformatics and experimental validation

Figure 1 Kinetochore-associated protein 1 expression in pan-cancer and gastric cancer tissues. A: Kinetochore-associated protein 1 (KNTC1) expression in pan-cancer tissue from The Cancer Genome Atlas and Genotype-Tissue Expression database; B and C: KNTC1 expressions in tumor and unpaired normal tissues (B) and paired adjacent normal tissues (C) from The Cancer Genome Atlas gastric cancer (GC) database; D and E: Immunohistochemical staining of KNTC1 in GC tissues and adjacent nontumorous tissues, with a representative image from five paired samples (scale bar = 100 μm). Immunohistochemical scoring was performed using the H-score system, where the staining intensity (IS) was graded from 0 to 3, and the percentage of positively stained tumor cells (AP) was graded from 0 to 4. The H-score was calculated as ∑(IS × AP); F: Expression of KNTC1 in GC was assessed through immunohistochemistry and western blotting; G: Expression of KNTC1 in GSE65801 (n = 32, T = 32) database; H: Area under the curve of KNTC1 in GC. ACC: Adrenocortical carcinoma; BLCA: Bladder urothelial carcinoma; BRCA: Breast invasive carcinoma; CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL: Cholangiocarcinoma; COAD: Colon adenocarcinoma; DLBC: Lymphoid neoplasm diffuse large B-cell lymphoma; ESCA: Esophageal carcinoma; GBM: Glioblastoma multiforme; HNSC: Head and neck squamous cell carcinoma; KICH: Kidney chromophobe; KIRC: Kidney renal clear cell carcinoma; KIRP: Kidney renal papillary cell carcinoma; LAML: Acute myeloid leukemia; LGG: Brain lower grade glioma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; LUSC: Lung squamous cell carcinoma; MESO: Mesothelioma; OV: Ovarian serous cystadenocarcinoma; PAAD: Pancreatic adenocarcinoma; PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; SARC: Sarcoma; SKCM: Skin cutaneous melanoma; STAD: Stomach adenocarcinoma; TGCT: Testicular germ cell tumors; THCA: Thyroid carcinoma; THYM: Thymoma; UCEC: Uterine corpus endometrial carcinoma; UCS: Uterine carcinosarcoma; UVM: Uveal melanoma; KNTC1: Kinetochore-associated protein 1; AUC: Area under the curve; CI: Confidence interval.