Contribution of the appendix to ulcerative colitis by supplying pathogenic immunoglobulin G-producing cells

Figure 6 Immunoglobulin G-positive cells express C-C chemokine receptor 10 in the appendices and rectums of patients with ulcerative colitis. A: C-C chemokine receptor 10 (CCR10) expression on uniform manifold approximation and projection of single-cell RNA sequencing data; B: Flow cytometric gating plots; CCR10 and immunoglobulin (Ig) G expression in appendiceal plasma cells; C: Immunohistochemistry for CCR10, IgG, and IgA in ulcerative colitis (UC) appendix. Top panel, bars = 100 μm; bottom (enlarged view of square in top panel), bars = 50 μm; D: Immunohistochemistry for CCR10, IgG, and IgA in the UC rectum. Top panel, bars = 100 μm; bottom (enlarged view of square in top panel), bars = 50 μm; E: Correlation between IgG-positive and CCR10-positive cells per immunohistochemistry field of the UC appendix. Spearman’s rank correlation. Ig: Immunoglobulin; UC: Ulcerative colitis; UMAP: Uniform manifold approximation and projection.