Contribution of the appendix to ulcerative colitis by supplying pathogenic immunoglobulin G-producing cells

Figure 2 Single-cell landscape of appendiceal lamina propria mononuclear cells in patients with ulcerative colitis and controls. A: Hematoxylin and eosin staining of the appendix of control (left) and ulcerative colitis (UC) (right) patient. Bars = 500 μm; B: Lamina propria mononuclear cells (LPMCs) per tissue weight in the appendix of controls (n = 10) and UC (n = 10). Data are shown as mean ± SEM; C: Experimental design of single-cell RNAsequencing analysis. Appendiceal LPMCs were isolated, and EpCAM^- and 7-amino-actinomycin D^- cells were sorted using FACSAria II and subjected to single-cell RNAsequencing; D: Uniform manifold approximation and projection of appendiceal LPMCs UC (n = 4) and controls (n = 5); E: Expression of genes across cell types; F: Frequencies of cell types in UC (n = 4) and controls (n = 5). Scatter plots with a median line. ILC: Innate lymphoid cell; LPMCs: Lamina propria mononuclear cells; NK: Natural killer; scRNA-seq: Single-cell RNAsequencing; UC: Ulcerative colitis; UMAP: Uniform manifold approximation and projection; 7-AAD: 7-amino-actinomycin D.