High cyclin-dependent kinase 5 expression promotes tumor progression in gastric neuroendocrine carcinoma

Figure 4 Downregulation of cyclin-dependent kinase 5 in gastric neuroendocrine carcinoma cells inhibits both cellular proliferation and colony formation. A: The expression levels of cyclin-dependent kinase 5 (CDK5) were analyzed in gastric neuroendocrine carcinoma and gastric cancer cell lines; B: ECC10 and ECC12 cell lines with stable CDK5 knockdown, alongside control cell lines, were established; C-E: The proliferation rates of cells with shCDK5 knockdown or treated with the CDK5 inhibitor roscovitine, as well as control cells, were evaluated using cell counting kit-8 and sulforhodamine B assays; F and G: Colony formation assays were conducted to evaluate the colony-forming capabilities of shCDK5 knockdown and control cells. ^aP < 0.05. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; CCK8: Cell count kit-8; SRB: Sulforhodamine B; CDK5: Cyclin-dependent kinase 5; NS: Not significant.