Calcium-dependent signaling protein family member S100A11 promotes liver and adipose lipid accumulation through fibroblast growth factor 21

Figure 8 S100A11 overexpression in hepatocytes promotes lipid accumulation in adipocytes. All data are presented as mean ± SE. n = 5 or 6 biological repeats. Statistical analysis was performed using unpaired two-tailed Student’s t test: ^aP < 0.05 vs recombinant adenoviruses encoding green fluorescent protein. Mouse-derived normal liver AML12 cells were cocultured with preadipocyte 3T3-L1 cells in vitro. When 3T3-L1 cells were approximately 70% confluent, adipogenic differentiation was performed, and recombinant adenoviruses encoding S100A11 were transfected into hepatocytes. Real-time polymerase chain reaction was used to detect related mRNA levels of AML12 cells and 3T3-L1 cells after 72 hours. A: MRNA level of S100A11 in AML12 cells; B: MRNA level of fibroblast growth factor 21 (FGF21) in AML12 cells; C: FGF21 level in AML12 cell culture supernatant; D: MRNA level of Fgf21 in 3T3-L1 cells; E and F: Expression levels of genes related to brown adipose tissue and white adipose tissue in 3T3-L1 cells. FGF21: Fibroblast growth factor 21; AD-GFP: Recombinant adenoviruses encoding green fluorescent protein; AD-SA/AD-S100A11: Recombinant adenoviruses encoding S100A11; UCP1: Uncoupling protein 1; PGC1α: Peroxisome proliferator-activated receptor gamma coactivator 1-α; PRDM16: PR domain containing 16.