Calcium-dependent signaling protein family member S100A11 promotes liver and adipose lipid accumulation through fibroblast growth factor 21

Figure 4 Adipose tissue and expression of fat-related gene mRNA in different tissues of male S100A11-hTg mice. All data are presented as mean ± SE. n = 6 mice/group. Statistical analysis was performed using one-way analysis of variance, P values were adjusted for multiple testing using the Bonferroni correction: ^aP < 0.05 vs normal-chow diet wild type, ^bP < 0.05 vs high-fat diet wild type. Five-week-old male S100A11-hTg mice and control mice were fed with normal-chow diet or high-fat diet for 12 weeks. A: General picture of subcutaneous fat at 17 weeks of age; B: General picture of perirenal fat at 17 weeks of age; C: General picture of epididymal fat at 17 weeks of age; D: At 17 weeks, the weight of adipose tissue in each region of the mice; E: Real-time polymerase chain reaction was used to detect expression of brown fat-related genes (left) and white fat-related genes (right) in white adipose tissue of the epididymis. F: Real time polymerase chain reaction was used to detect the expression level of brown fat-related genes (left) and white fat-related genes (right) in brown adipose tissue. Scale bar: 1 cm. NCD: Normal-chow diet; HFD: High-fat diet; WT: Wild type; sWAT: Subcutaneous white adipose tissue; eWAT: Epididymal white adipose tissue; rWAT: Retroperitoneal white adipose tissue; BAT: Brown adipose tissue; UCP1: Uncoupling protein 1; PGC1α: Peroxisome proliferator-activated receptor gamma coactivator 1-α; PRDM16: PR domain containing 16; PAI-1: Plasminogen activator inhibitor-1.