Calcium-dependent signaling protein family member S100A11 promotes liver and adipose lipid accumulation through fibroblast growth factor 21

Figure 2 High-fat diet increased liver triglyceride levels in male S100A11-hTg mice. All data are presented as mean ± SE. n = 5-6 mice/group. Statistical analysis was performed using one-way analysis of variance, P values were adjusted for multiple testing using the Bonferroni correction: ^aP < 0.05 vs normal-chow diet wild type, ^bP < 0.05 vs high-fat diet wild type. Five-week-old male S100A11-hTg mice and littermate control mice were fed normal-chow diet or high-fat diet for 12 weeks. A: General liver image of mice at 17 weeks of age, scale bar: 1 cm; B: Triglyceride concentration in the liver of mice at 17 weeks of age; C: Real-time polymerase chain reaction (PCR) was used to detect expression of hepatic lipid synthesis-related genes; D: Real-time PCR was used to detect expression of hepatic lipid uptake-related genes; E: Real-time PCR was used to detect expression of hepatic lipid oxidation-related genes; F: Real-time PCR was used to detect expression of hepatic cholesterol synthesis-related genes; G: Oil Red O staining of mouse liver and quantification of positive areas, scale bar: 100 μm. NCD: Normal-chow diet; HFD: High-fat diet; WT: Wild type; ACC: Acetyl-CoA carboxylase; DGAT: Diacylglycerol acyltransferase; FASN: Fatty acid synthase; PPAR: Peroxisome proliferator-activated receptor; Cpt1α: Carnitine palmitoyltransferase 1α; MCADH: Medium-chain acyl-CoA dehydrogenase; HMGCR: 3-hydroxy-3-methyl-glutaryl-CoA reductase; SREBP2: Sterol regulatory element-binding protein 2; HMGCS1: 3-hydroxy-3-methylglutaryl-CoA synthase 1.