Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 8 Dendrobium officinale extract mediates the regulation of the “InR-Nrf2” signaling axis by gut microbiota through gender dimorphism, in order to combat oxidative stress and extend healthy lifespan. A: Schematic diagram illustrating the potential mechanism of Dendrobium officinale extract (DOE) differential regulation of dominant bacterial species in both sexes; B: Relative expression levels of InR in flies with or without DOE (n = 3); C: Relative expression levels of Dilp2 in flies with or without DOE (n = 3); D: Relative expression levels of Dilp3 in flies with or without DOE (n = 3); E: Relative expression levels of InR in flies after intervention with Acetobacter sp. or Lactobacillus sp. (n = 3); F: Relative expression levels of Dilp2 in flies after intervention with Acetobacter sp. or Lactobacillus sp. (n = 3); G: Relative expression levels of Dilp3 in flies after intervention with Acetobacter sp. or Lactobacillus sp. (n = 3); H: Survival curves for Da-gal4 > UAS-InR RNAi with or without DOE treatment (n = 150 files); I: Intestinal integrity in Da-gal4 > UAS-InR RNAi with or without DOE (n = 3); J: Reactive oxygen species level in Da-gal4 > UAS-InR RNAi with or without DOE (n = 3); K: Relative expression of Keap1-Nrf2-antioxidant response element pathway-related genes in Da-gal4 > UAS-InR RNAi with or without DOE (n = 3); L: Relative expression levels of InR, Dilp2, Dilp3 in Da-gal4 > UAS-CncC RNAi with or without DOE (n = 3). Data were analyzed by log-rank test (survival) or one/two-way analysis of variance with post-hoc test (multiple comparisons). ^aP < 0.05 vs control group. ^bP < 0.01 vs control group. ^cP < 0.001 vs control group. ^dP < 0.0001 vs control group. DOE: Dendrobium officinale extract; mRNA: Messenger RNA; CAT: Catalase; SOD: Superoxide dismutase; ROS: Reactive oxygen species; CuZn: Copper zinc; Mn: Manganese; MTH: Methuselah; GclC: Glutamate-cysteine ligase catalytic subunit.