Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 7 Midlife Intervention with Acetobacter sp. and Lactobacillus sp. mitigates oxidative stress, fosters intestinal homeostasis, and extends healthy lifespan. A: Schematic diagram of advantageous bacterial species replanting; B: Survival curve of early, midlife, late and lifelong intervention with Acetobacter sp. in female flies (n = 150 flies); C: Survival curve of early, midlife, late and lifelong intervention with Lactobacillus sp. in male flies (n = 150 flies); D: Survival curves for wild type (WT) or sterile female flies with or without dominant strain treatment (n = 150 flies); E: Survival curves for WT or sterile male flies with or without dominant strain treatment (n = 150 flies); F: Climbing number in WT or sterile flies with or without dominant strain treatment (n = 3); G and H: Intestinal leakage in WT or sterile flies with or without dominant strain treatment (n = 3); I: Activity of catalase in WT or sterile flies with or without dominant strain treatment (n = 3); J: Activity of superoxide dismutase in WT or sterile flies with or without dominant strain treatment (n = 3); K: Activity of malondialdehyde in WT or sterile flies with or without dominant strain treatment (n = 3); L: Reactive oxygen species level in WT or sterile flies with or without dominant strain treatment (n = 3). The data are expressed as the mean ± SD. Data were analyzed by log-rank test (survival) or one-way analysis of variance with post-hoc test (multiple comparisons). ^aP < 0.05 vs control group. ^bP < 0.01 vs control group. ^cP < 0.05 vs wild type group. ^dP < 0.01 vs wild type group. ^eP < 0.001 vs wild type group. ^fP < 0.05 vs sterile group. ^gP < 0.01 vs sterile group. ^hP < 0.001 vs sterile group. CAT: Catalase; SOD: Superoxide dismutase; MDA: Malondialdehyde; ROS: Reactive oxygen species; WT: Wild type.