Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 6 Dendrobium officinale extract antagonizes oxidative stress and prolongs healthy lifespan dependent on Nrf2. A and B: Survival curves of EU1 nematodes (n = 90 nematodes); C: Intestinal atrophy of EU1 with or without Dendrobium officinale extract (DOE) (n = 3); D: Intestinal integrity of EU1 with or without DOE (n = 3); E: Reactive oxygen species (ROS) level of EU1 with or without DOE (n = 3); F: Antioxidant gene expression of EU1 with or without DOE (n = 3); G: Image showing the construction process of Da-gal4 > UAS-CncC RNAi; H: Survival curves for CncC mutant flies (n = 150 flies); I: Intestinal integrity in CncC mutant flies with or without DOE (n = 3); J: ROS level in CncC mutant flies with or without DOE (n = 3); K: Antioxidant gene expression in CncC mutant flies with or without DOE (n = 3). The data are expressed as the mean ± SD. Data were analyzed by log-rank test (survival), Student’s t-test (two groups) or two-way analysis of variance with post-hoc test (multiple comparisons). DOE: Dendrobium officinale extract; CAT: Catalase; SOD: Superoxide dismutase; ROS: Reactive oxygen species; CuZn: Copper zinc; Mn: Manganese; MTH: Methuselah; GclC: Glutamate-cysteine ligase catalytic subunit.