Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 3 Dendrobium officinale extract midlife intervention extends healthy lifespan. A: Motility in nematodes after staged Dendrobium officinale extract (DOE) intervention (n = 90 nematodes); B: Pumping frequency in nematodes after staged DOE intervention (n = 90 nematodes); C and D: Intestinal atrophy in nematodes after staged DOE intervention (n = 90 nematodes); E and F: Intestinal leakage in nematodes after staged DOE intervention (n = 90 nematodes); G and H: Microbial colonization capacity in nematodes after staged DOE intervention (n = 90 nematodes); I: Food intake in flies after staged DOE intervention (n = 3); J: Reproductivity in flies after staged DOE intervention (n = 3); K: Climbing number in flies after staged DOE intervention (n = 3); L and M: Intestinal integrity in flies after staged DOE intervention (n = 3). The data are expressed as the mean ± SD. Data were analyzed by one-way analysis of variance with post-hoc test (multiple comparisons). ^aP < 0.05 vs control group. ^bP < 0.01 vs control group. ^cP < 0.001 vs control group. ^dP < 0.0001 vs control group.