Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 2 Midlife is the best intervention window for Dendrobium officinale extract to extend lifespan. A: Dendrobium officinale extract (DOE) intervention duration screening strategy for nematodes; B: DOE intervention duration screening strategy for flies; C: Survival curve of DOE treatment for 4 days in nematodes (n = 90 nematodes); D: Survival curve of DOE treatment for 20 days in female flies (n = 150 flies); E: Survival curve of DOE treatment for 20 days in male flies (n = 150 flies); F: Comparison of the average lifespan extension rate of nematodes after 2 days, 4 days, 8 days, and lifelong treatment with DOE (n = 3); G: Comparison of the lifespan extension rate of female flies after 10 days, 20 days, 30 days, 40 days, and lifelong treatment with DOE (n = 3); H: Comparison of the lifespan extension rate of male flies after 10 days, 20 days, 30 days, 40 days, and lifelong treatment with DOE (n = 3); I: Staged DOE intervention strategies in nematodes; J: Staged DOE intervention strategies in flies; K: Survival curve of DOE midlife intervention for 4 days in nematodes (n = 90 nematodes); L: Survival curve of DOE midlife intervention for 20 days in female flies (n = 150 flies); M: Survival curve of DOE midlife intervention for 20 days in male flies (n = 150 flies); N: Comparison of the mean lifespan after intervention with DOE in the early, midlife, late, and lifelong stages in nematodes (n = 3); O: Comparison of the mean lifespan after intervention with DOE in the early, midlife, late, and lifelong stages in female flies (n = 3); P: Comparison of the mean lifespan after intervention with DOE in the early, midlife, late, and lifelong stages in male flies (n = 3). The data are expressed as the mean ± SD. Data were analyzed by log-rank test (survival) or one-way analysis of variance with post-hoc test (multiple comparisons). ^aP < 0.05 vs control group in nematodes. ^bP < 0.01 vs control group in flies. ^cP < 0.0001 vs 2-day group in nematodes. ^dP < 0.0001 vs 10-day group in flies. ^eP < 0.01 vs control group in nematodes with midlife intervention. ^fP < 0.001 vs control group in flies with midlife intervention. ^gP < 0.001 vs midlife group in nematodes. ^hP < 0.0001 vs midlife group in nematodes. ⁱP < 0.01 vs midlife group in flies. ^jP < 0.001 vs midlife group in flies. ^kP < 0.001 vs midlife group in flies. DOE: Dendrobium officinale extract.