Midlife intervention of Dendrobium officinale extract modulates gut microbiota to activate InR-Nrf2 axis, promoting intestinal health and longevity

Figure 1 Survival of nematodes, flies, and MRC-5 cells after supplementation with medium containing Dendrobium officinale extract. A: Survival curve of nematodes (n = 90 nematodes); B: Mean lifespan of nematodes (n = 90 nematodes); C: Survival curve for female flies (n = 150 flies); D: Mean lifespan for female flies (n = 150 flies); E: Survival curve for male flies (n = 150 flies); F: Mean lifespan for male flies (n = 150 flies); G: Effect on viability of MRC-5 cells induced by Dendrobium officinale extract (DOE) by cell counting kit-8 (CCK-8) assay (n = 3); H: Effect on viability of MRC-5 cells induced by hydrogen peroxide by CCK-8 assay (n = 3); I: CCK-8 assays of damaged MRC-5 cells treated with DOE (n = 3); J: Aging-related galactosidase staining (SA-β-gal), senescent cells are stained dark blue-green, and have increased cell volume and irregular shapes, scale bar = 200 μm. The data are expressed as the mean ± SD. Data were analyzed by log-rank test (survival) or one-way analysis of variance with post-hoc test (multiple comparisons). ^aP < 0.05 vs control group in nematodes. ^bP < 0.001 vs control group in nematodes. ^cP < 0.001 vs control group in female flies. ^dP < 0.01 vs control group in female flies. ^eP < 0.01 vs control group in male flies. ^fP < 0.01 vs control group in male flies. ^gP < 0.05 vs control group in MRC-5 cells. ^hP < 0.01 vs control group in MRC-5 cells. ⁱP < 0.001 vs control group in MRC-5 cells. ^jP < 0.0001 vs control group in MRC-5 cells. ^kP < 0.05 vs model group in MRC-5 cells. C. elegans: Caenorhabditis elegans; DOE: Dendrobium officinale extract; H₂O₂: Hydrogen peroxide; con: Control.