Human epidermal growth factor receptor 2 in combined hepatocellular-cholangiocarcinoma: Prognostic and predictive guide for adjuvant chemotherapy

Figure 3 Postoperative analysis of tertiary lymphoid structures in patient tissue specimens by immunofluorescence. A: High-magnification view showing the spatial distribution and density of CD20⁺ B cells within the tertiary lymphoid structure (TLS); B: High-magnification view showing the spatial distribution and density of CD4⁺ T helper cells; C: High-magnification view showing the spatial distribution and density of CD8⁺ cytotoxic T cells; D: High-magnification view showing the spatial distribution and density of the CD21⁺ follicular dendritic cell network; E: Visualization of tissue architecture and cell nuclei using DAPI nuclear counterstain; F: Merged image showing the integrated architecture and spatial co-localization of the various immune cell subsets within a representative TLS.