Tropomyosin 3 regulates tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma to promote malignant progression in gastric cancer

Figure 7 Tropomyosin 3 influences cell cycle, apoptosis, matrix metalloproteinase family proteins, and epithelial-mesenchymal transition in gastric cancer cells through tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, and activates the mitogen-activated protein kinase signaling pathway. A and B: Western blotting analysis confirmed that tropomyosin 3 influences the cell cycle, apoptosis, matrix metalloproteinase family proteins, and epithelial-mesenchymal transition in gastric cancer cells through tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, and activates the mitogen-activated protein kinase signaling pathway; C: Western blotting analysis of HGC27 cells overexpressing tropomyosin 3, followed by tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma knockdown or treatment with mitogen-activated protein kinase pathway inhibitors, including SCH772984 (extracellular signal-regulated kinase inhibitor, 20 μM), SB203580 (p38 inhibitor, 20 μM), and SP600125 (c-Jun N-terminal kinase inhibitor, 20 μM). ERK: Extracellular signal-regulated kinase; JNK: C-Jun N-terminal kinase; OE: Overexpression plasmid; MMP: Matrix metalloproteinase; TPM3: Tropomyosin 3; YWHAG: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma.