Tropomyosin 3 regulates tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma to promote malignant progression in gastric cancer

Figure 5 Tropomyosin 3 interacts with tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma in gastric cancer cells and positively regulates the expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma. A: Tropomyosin 3 (TPM3) was significantly positively correlated with tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma (YWHAG) in The Cancer Genome Atlas cohort from GEPIA 2.0 database; B: Gene Expression Omnibus databases (GSE54129 and GSE66229) analysis of YWHAG mRNA expression in normal tissues and gastric cancer (GC) tissues; C: Comparative analysis of the expression differences of YWHAG in normal tissues and GC tissues using the GEPIA2.0 database; D: Quantitative real-time PCR analysis of YWHAG mRNA expression in 62 pairs of tumorous and para-carcinoma tissue from the Second Affiliated Hospital of Nanchang University; E: Western blotting showed the protein expression of YWHAG in tumorous and para-carcinoma tissue; F: Co-immunoprecipitation indicated the presence of direct or indirect binding between TPM3 and YWHAG; G: The colocalization between TPM3 and YWHAG was visualized by a confocal laser scanning microscope in different GC cell lines; H: Western blotting analysis was performed to observe the expression changes of YWHAG following TPM3 knockdown or overexpression; I: Quantitative real-time PCR analysis was performed to examine the expression changes of YWHAG following TPM3 knockdown or overexpression. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. NS: Not significant; Co-IP: Co-immunoprecipitation; IgG: Immunoglobulin G; sh-NC: Control shRNA; STAD: Stomach adenocarcinoma; TPM3: Tropomyosin 3; YWHAG: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; Vector: Empty vector control.