Tropomyosin 3 regulates tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma to promote malignant progression in gastric cancer

Figure 3 Tropomyosin 3 facilitates cell cycle progression and suppresses apoptosis in gastric cancer cells. A: Flow cytometry analysis of cell cycle distribution in AGS and HGC27 cells following tropomyosin 3 knockdown or overexpression; B: Quantification of cell cycle phase distribution (G1, S, and G2) in AGS and HGC27 cells; C: Flow cytometry analysis of apoptosis using annexin V fluorescein isothiocyanate/propidium iodide staining in AGS and HGC27 cells following tropomyosin 3 knockdown or overexpression; D: Quantification of apoptosis rate in AGS and HGC27 cells; E: Western blotting analysis of proteins associated with cell cycle and apoptosis; F: Western blotting analysis was performed to examine the expression levels of epithelial-mesenchymal transition-related proteins and matrix metalloproteinase family proteins. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. NS: Not significant; FITC: Fluorescein isothiocyanate; OE: Overexpression plasmid; sh-NC: Control shRNA; TPM3: Tropomyosin 3; Vector: Empty vector control.