Tropomyosin 3 regulates tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma to promote malignant progression in gastric cancer

Figure 1 Tropomyosin 3 is upregulated in gastric cancer sample and associated with poor prognosis. A: Differential expression analysis of tropomyosin 3 (TPM3) in multiple tumors from Tumor Immune Estimation Resource database; B: Comparative analysis of the expression differences of TPM3 in normal tissues and gastric cancer (GC) tissues using the GEPIA2.0 database; C: Gene Expression Omnibus databases (GSE54129 and GSE66229) analysis of TPM3 mRNA expression in normal tissues and GC tissues; D: Analyze the difference in overall survival between the high-expression group and the low-expression group of TPM3 on the Kaplan-Meier plotter website; E: Quantitative real-time PCR analysis of TPM3 mRNA expression in 62 pairs of tumorous and para-carcinoma tissue from the Second Affiliated Hospital of Nanchang University; F: Western blotting showed the protein expression of TPM3 in tumorous and para-carcinoma tissue (it shows that TPM3 protein expression in GC tissues presents as dual bands, representing different subtypes); G: Immunohistochemistry showed the protein expression of TPM3 in tumorous and para-carcinoma tissue; H: Overall survival of GC patients with low or high levels of TPM3; I: Protein and mRNA expression of TPM3 in GC cell lines. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. HR: Hazard ratio; STAD: Stomach adenocarcinoma; TPM3: Tropomyosin 3.