Growth arrest specific 6 ameliorated inflammation and portal pressure through activating efferocytosis in cholestasis and portal hypertension

Figure 1 Liver injury and portal hypertension in bile duct ligation- and 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestasis. A: The portal pressure of mice among four groups; B: The representative images of liver sections stained with hematoxylin & eosin (H&E), Sirius Red, Masson, or by immunohistochemical with primary antibody against CK19 among four groups; C: The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL), and total bile acid (TBA) in sham and bile duct ligation (BDL) mice; D: The survival curve of mice after sham or BDL operation; E: The serum levels of ALT, AST, TBIL, DBIL, and TBA in normal chow diet and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) mice; F: The survival curve of mice fed with DDC diet; G: The quantifications of necrotic area in H&E staining, Masson- or Sirius Red-stained area, and numbers of CK19⁺ cholangiocytes. ^aP < 0.05, ^bP < 0.01. H&E: Hematoxylin & eosin; NCD: Normal chow diet; DDC: Diethoxycarbonyl-1,4-dihydrocollidine; BDL: Bile duct ligation.