MFSD2A suppresses CD8+ T cell exhaustion in colorectal cancer by inhibiting ERK/p38 MAPK-PD-L1 signaling

Figure 5 Major facilitator superfamily domain containing 2A overexpression inhibits tumor growth and promotes CD8+ T cell infiltration, concurrent with suppressed mitogen-activated protein kinase signaling in vivo. A: Subcutaneous tumor growth following injection of MC38, overexpression negative control MC38, or major facilitator superfamily domain containing 2A-overexpressing MC38 cells in C57BL/6 mice; B: Hematoxylin and eosin staining; C: Ki67 immunohistochemistry of tumor tissue; D: Western blotting analysis of p-extracellular signal-regulated kinase 1/2, extracellular signal-regulated kinase 1/2, p-p38 mitogen-activated protein kinase, and p38 mitogen-activated protein kinase expression in tumor tissue; E: Immunofluorescence staining of CD8+ T cell infiltration in tumor tissue. oeMFSD2A: Major facilitator superfamily domain containing 2A-overexpressing; oeNC: Overexpression negative control; HE: Hematoxylin and eosin; ERK: Extracellular signal-regulated kinase; MAPK: Mitogen-activated protein kinase.