MFSD2A suppresses CD8+ T cell exhaustion in colorectal cancer by inhibiting ERK/p38 MAPK-PD-L1 signaling

Figure 4 Major facilitator superfamily domain containing 2A suppresses CD8+ T cell exhaustion via the extracellular signal-regulated kinase/p38 mitogen-activated protein kinase signaling pathway. A: Western blotting analysis of programmed cell death protein 1 and cytotoxic T lymphocyte-associated antigen 4 expression in CD8+ T cells following co-culture with MC38 cells treated with or without epidermal growth factor (EGF); B: Killing assay (24 hours) showing lysis rates of major facilitator superfamily domain containing 2A-overexpressing MC38 cells co-cultured with CD8+ T cells in the presence or absence of EGF; C: Enzyme-linked immunosorbent assay quantification of interleukin-2, interferon-γ, and tumor necrosis factor-α secretion by CD8+ T cells incubated with major facilitator superfamily domain containing 2A-overexpressing MC38 cells under EGF-treated or untreated conditions. ^aP < 0.0001 vs major facilitator superfamily domain containing 2A-overexpressing. oeMFSD2A: Major facilitator superfamily domain containing 2A-overexpressing; EGF: Epidermal growth factor; PD1: Programmed cell death protein 1; CTLA4: Cytotoxic T lymphocyte-associated antigen 4; IL-2: Interleukin-2; IFN-γ: Interferon-γ; TNF-α: Tumor necrosis factor-α.