MFSD2A suppresses CD8+ T cell exhaustion in colorectal cancer by inhibiting ERK/p38 MAPK-PD-L1 signaling

Figure 3 Major facilitator superfamily domain containing 2A suppresses malignant phenotypes and programmed death-ligand 1 expression in colorectal cancer cells via the extracellular signal-regulated kinase/p38 mitogen-activated protein kinase pathway. A: Western blotting analysis of major facilitator superfamily domain containing 2A (MFSD2A), p-extracellular signal-regulated kinase 1/2, extracellular signal-regulated kinase 1/2, p-p38 mitogen-activated protein kinase, and p38 mitogen-activated protein kinase expression in MFSD2A-overexpressing MC38 cells treated with or without epidermal growth factor (EGF); B: Cell counting kit-8 assay was performed on MC38 cells with or without EGF (50 ng/mL) treatment; C: Apoptosis assay was performed on MC38 cells with or without EGF (50 ng/mL) treatment; D: Wound healing assay was used to assess the effects of EGF on MC38 cell migration; E: Transwell assay was used to assess the effects of EGF on MC38 cell invasion; F: Western blotting analysis of programmed death-ligand 1 expression in MFSD2A-overexpressing MC38 cells treated with or without EGF (50 ng/mL). ^aP < 0.0001 vs control, ^bP < 0.0001 vs major facilitator superfamily domain containing 2A-overexpressing, ^cP < 0.01 vs major facilitator superfamily domain containing 2A-overexpressing, and ^dP < 0.001 vs Major facilitator superfamily domain containing 2A-overexpressing. oeMFSD2A: Major facilitator superfamily domain containing 2A-overexpressing; MFSD2A: Major facilitator superfamily domain containing 2A; ERK: Extracellular signal-regulated kinase; MAPK: Mitogen-activated protein kinase; EGF: Epidermal growth factor; PD-L1: Programmed death-ligand 1.