MFSD2A suppresses CD8+ T cell exhaustion in colorectal cancer by inhibiting ERK/p38 MAPK-PD-L1 signaling

Figure 1 Major facilitator superfamily domain containing 2A inhibits the malignant progression of colorectal cancer cells in vitro. A: Western blotting analysis of major facilitator superfamily domain containing 2A (MFSD2A) expression in normal colonic epithelial cells, Caco-2, SW480, and MC38 cells; B: Validation of MFSD2A overexpression and knockdown by western blotting; C: The relative MFSD2A expression level in cells was determined by quantitative polymerase chain reaction; D: Cell proliferation was determined using the cell counting kit-8 assay; E and F: Apoptosis detected using allophycocyanin Annexin V/propidium iodide flow cytometry; G and H: Wound healing assays were performed to evaluate the migration capability of MFSD2A-overexpressing MC38 and MFSD2A-knockdown Caco-2 cells; I and J: Transwell assays were performed to evaluate the invasion capability of MFSD2A-overexpressing MC38 and MFSD2A-knockdown Caco-2 cells. ^aP < 0.0001 vs overexpression negative control, ^bP < 0.01 vs overexpression negative control, ^cP < 0.0001 vs siRNA negative control, and ^dP < 0.01 vs siRNA negative control. MFSD2A: Major facilitator superfamily domain containing 2A; NCM460: Normal colonic epithelial cells; oeMFSD2A: Major facilitator superfamily domain containing 2A-overexpressing; oeNC: Overexpression negative control; siMFSD2A: Major facilitator superfamily domain containing 2A-knockdown; siNC: SiRNA negative control.