PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma

Figure 3 PRMT5 suppressed DDIT3 transcription by increasing H4R3me2 modification at the DDIT3 promoter. A and B: The effect of PRMT5 overexpression and knockdown on DDIT3 promoter activity in hepatocellular carcinoma (HCC) cells; C: Location diagram of designed chromatin immunoprecipitation (ChIP) primers on DDIT3 promoter; D: ChIP examined the enrichment of PRMT5 on DDIT3 promoter; E and F: The level of H4R3me2 of DDIT3 promoter was evaluated in PRMT5 overexpression and knockdown HCC cells by ChIP assay. Data are presented as the mean ± SD. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. NS: Not significant; Scr: Scramble; IgG: Immunoglobulin G; shPRMT5: Short hairpin RNA of PRMT5.