PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma

doi:10.3748/wjg.v32.i19.115332

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World Journal of Gastroenterology

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1007-9327

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastroenterol. May 21, 2026; 32(19): 115332
Published online May 21, 2026. doi: 10.3748/wjg.v32.i19.115332

Table 2 Primers of short hairpin RNA targeted to PRMT5 and DDIT3

Primer	Sequence
shPRMT5-1	F: 5’-CCGGAGTACCAGCAGGCCATCTATACTCGAGTATAGATGGCCTGCTGGTACTTTTTTG-3’
shPRMT5-1	R: 5’-AATTCAAAAAAGTACCAGCAGGCCATCTATACTCGAGTATAGATGGCCTGCTGGTACT-3’
shPRMT5-2	F: 5’-CCGGGCCCAGTTTGAGATGCCTTATCTCGAGATAAGGCATCTCAAACTGGGCTTTTTG-3’
shPRMT5-2	R: 5’-AATTCAAAAAGCCCAGTTTGAGATGCCTTATCTCGAGATAAGGCATCTCAAACTGGGC-3’
shDDIT3-1	F: 5’-CCGGCTGCACCAAGCATGAACAATTCTCGAGAATTGTTCATGCTTGGTGCAGTTTTTG-3’
shDDIT3-1	R: 5’-AATTCAAAAACTGCACCAAGCATGAACAATTCTCGAGAATTGTTCA TGCTTGGTGCAG-3’
shDDIT3-2	F: 5’-CCGGTGAACGGCTCAAGCAGGAAATCTCGAGATTTCCTGCTTGAGCCGTTCATTTTTG-3’
shDDIT3-2	R: 5’-AATTCAAAAATGAACGGCTCAAGCAGGAAATCTCGAGATTTCCTGCTTGAGCCGTTCA-3’

shPRMT5: Short hairpin RNA of PRMT5; shDDIT3: Short hairpin RNA of DDIT3; F: Forward primer; R: Reverse primer.

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Citation: Jiang H, Yan JH, Tang WJ, Shen B, Mo S, Wang Y, Hu DH, Dong ZX, Zhang SB. PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma. World J Gastroenterol 2026; 32(19): 115332
URL: https://www.wjgnet.com/1007-9327/full/v32/i19/115332.htm
DOI: https://dx.doi.org/10.3748/wjg.v32.i19.115332

Jiang H, Yan JH, Tang WJ, Shen B, Mo S, Wang Y, Hu DH, Dong ZX, Zhang SB. PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma. World J Gastroenterol 2026; 32(19): 115332 [DOI: 10.3748/wjg.v32.i19.115332]

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