PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma

doi:10.3748/wjg.v32.i19.115332

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastroenterol. May 21, 2026; 32(19): 115332
Published online May 21, 2026. doi: 10.3748/wjg.v32.i19.115332

Table 1 Primers of cloning genes and real-time quantitative polymerase chain reaction

Primer	Sequence
PRMT5 qPCR	F: 5’-CGATCAGACCTACTGCTGTCA-3’
PRMT5 qPCR	R: 5’-CTCGGAGTTCCTGCGAATCT-3’
DDIT3 qPCR	F: 5’-CTCGCTCTCCAGATTCCAGTC-3’
DDIT3 qPCR	R: 5’-CTTCATGCGTTGCTTCCCA-3’
DDIT3 promoter	F: 5’-CCCTCGAGTCATTTTTAAAGAATGAGTTAAGGG-3’
DDIT3 promoter	R: 5’-GATATCTGGGGAATGACCACTCTGTTTCC-3’
PRMT5 clone	F: 5’-CGGGATCCATGGCGGCGATGGCGGTC-3’
PRMT5 clone	R: 5’-CGACGCGTCGAGGCCAATGGTATATGAGCG-3’
DDIT3 clone	F: 5’-GGAATTCATGGAGCTTGTTCCAGCCACT-3’
DDIT3 clone	R: 5’-CGGGATCCTCATGCTTGGTGCAGATTCACCAT-3’

qPCR: Quantitative polymerase chain reaction; F: Forward primer; R: Reverse primer.

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Citation: Jiang H, Yan JH, Tang WJ, Shen B, Mo S, Wang Y, Hu DH, Dong ZX, Zhang SB. PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma. World J Gastroenterol 2026; 32(19): 115332
URL: https://www.wjgnet.com/1007-9327/full/v32/i19/115332.htm
DOI: https://dx.doi.org/10.3748/wjg.v32.i19.115332

Jiang H, Yan JH, Tang WJ, Shen B, Mo S, Wang Y, Hu DH, Dong ZX, Zhang SB. PRMT5 imposed a dual repression on DDIT3 transcription to promote the malignancy of hepatocellular carcinoma. World J Gastroenterol 2026; 32(19): 115332 [DOI: 10.3748/wjg.v32.i19.115332]

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