Serca2 deletion in the mouse adult Bmi1+ compartment induces a lethal phenotype involving a severe gastric dysfunction

Figure 5 Evaluation of conditional Serca2 deletion in adult bone marrow. A: Bone marrow (BM) cellularity in samples from DSerca2^Bmi1homo [10 days post-tamoxifen (Tx) induction] and control (Bmi1-GFP) animals (n = 2 each); B: Flow cytometry analysis of different BM populations (defined as in Supplementary Figure 6) in DSerca2^Bmi1homo (10 days post-Tx) and control animals (n = 2 each); C and D: Flow cytometry analysis of relevant T (C) and B (D) cell populations in DSerca2^Bmi1homo mice (10 days post-Tx) and control animals (n = 2 each); E and F: Evaluation of the immature Lin^- Sca1⁺ ckit⁺ (LSK) population representation (per femur) (E) and its in vivo rate of proliferation (5-ethynyl-2’-deoxyuridine +) (F) in DSerca2^Bmi1homo LSK (10 days post-Tx) and control LSK samples (n = 3 each; age-matched animals). Assays were performed two times and data are expressed as mean ± SD. ^aP < 0.05. The online version of this article includes the characterization, by flow cytometry, of the GFP⁺ population (Bmi1-GFP) distribution in bone marrow. RBC: Red blood cell; TCR: T-cell receptor; NK: Natural killer; CD: Cluster of differentiation; IgM: Immunoglobulin M; EdU: 5-ethynyl-2’-deoxyuridine.