Qiweizhigan granule ameliorates metabolic dysfunction-associated steatohepatitis by modulating the galectin 3/tumor necrosis factor receptor-associated factor 6-mediated ferroptosis

Figure 5 Galectin 3 regulates the inflammatory response in RAW264. 7 cells. A-C: The mRNA levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) in RAW264.7 cells following 24-hour intervention with lipopolysaccharide (LPS) (100 ng/mL) were significantly increased; D: Galectin 3 (LGALS3) mRNA expression in RAW264.7 cells after LPS intervention was significantly increased; E: Secreted LGALS3 level in RAW264.7 cell supernatant after LPS intervention was significantly increased; F and G: The mRNA levels of IL-1β, and IL-6 in RAW264.7 cells after intervention with recombinant LGALS3 protein were significantly increased; H and I: Secretion levels of IL-6 and TNF-α in RAW264.7 cells after recombinant LGALS3 protein intervention were significantly increased; J: The overexpression efficiency of LGALS3 in RAW264.7 cells transfected with LGALS3 overexpression plasmid was verified; K-M: The mRNA levels of IL-1β, IL-6, and TNF-α in RAW264.7 cells after LGALS3 overexpression were significantly increased; N and O: Secretion levels of IL-6 and TNF-α in RAW264.7 cells after LGALS3 overexpression were significantly increased. Data were presented as means ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. IL: Interleukin; LGALS3: Galectin 3; TNF-α: Tumor necrosis factor-α.