Qiweizhigan granule ameliorates metabolic dysfunction-associated steatohepatitis by modulating the galectin 3/tumor necrosis factor receptor-associated factor 6-mediated ferroptosis

Figure 3 Transcriptomics highlights galectin 3 as a key gene mediating the therapeutic effect of Qiweizhigan granule on metabolic dysfunction-associated steatohepatitis. A: Volcano plots between metabolic dysfunction-associated steatohepatitis model and control; B: Volcano plots between Qiweizhigan granule treated and metabolic dysfunction-associated steatohepatitis model groups. Orange dots indicate up-regulated differentially expressed genes (DEGs), green dots indicate down-regulated DEGs, and gray dots indicate no significant changes; C: Venn diagram between pairwise groups; D: Hierarchical cluster heatmap, green color indicates down-regulated and orange color indicates up-regulated; E: Top 25 enriched Gene Ontology terms, the X-axis indicates enrichment ratio, and the Y-axis indicates names of Gene Ontology term, the size and color of the dots indicates enriched gene counts and -log10 (P value), respectively; F: Top 25 enriched Kyoto Encyclopedia of Genes and Genomes pathways, the X-axis indicates enrichment ratio, and the Y-axis indicates Kyoto Encyclopedia of Genes and Genomes pathway names, the size and color of the dots indicates enriched gene counts and -log10 (P value), respectively. CDAHFD: Choline-deficient, L-amino acid-defined high-fat diet; LGALS3: Galectin 3; QWZG: Qiweizhigan granule.