Qiweizhigan granule ameliorates metabolic dysfunction-associated steatohepatitis by modulating the galectin 3/tumor necrosis factor receptor-associated factor 6-mediated ferroptosis

Figure 2 Qiweizhigan granule reduced fibrosis in metabolic dysfunction-associated steatohepatitis mice. A: Serum alanine aminotransferase level; B: Serum aspartate aminotransferaselevel; C: Serum lactate dehydrogenase level; D: Serum total bilirubin level; E: Serum triglycerides level; F: Serum total cholesterol level were performed among the six groups; G: Representative images of Masson’s trichrome staining and immunohistochemical staining of α-smooth muscle actin were shown among the three groups; H and I: Fibrosis score and western blots analysis of α-smooth muscle actin and β-actin protein expression were performed. Data were presented as means ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; α-SMA: α-smooth muscle actin; CDAHFD: Choline-deficient, L-amino acid-defined high-fat diet; LDH: Lactate dehydrogenase; QWZG: Qiweizhigan granule; TBIL: Total bilirubin; TC: Total cholesterol; TG: Triglycerides.