MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 10  The combination of Xiangshaliujunzi decoction and 5-fluorouracil promoted miR-200a/ZEB1/IRF1-mediated PANoptosis in transplanted tumor tissues in mice. The combination treatment group [5-fluorouracil (5-FU) + Xiangshaliujunzi decoction (XSLJZD)] showed higher levels of miR-200a-3p and IRF1 mRNA and lower ZEB1 mRNA compared to the individual 5-FU and XSLJZD groups. The combination treatment group (5-FU + XSLJZD) showed further increased levels of PANoptosis-related proteins and inflammatory cytokines [interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-18] compared to the 5-FU and XSLJZD groups individually. A: MiR-200a-3p, IRF1 mRNA and lower ZEB1 mRNA expression; B: PANoptosis-related proteins expression; C: IFN-γ, TNF-α, IL-1β, and IL-18 expression. ^aP < 0.05, ^bP < 0.01 vs control/sh-negative control. ^dP < 0.05, ^fP < 0.001 vs 5-FU. ^gP < 0.05 vs XSLJZD. XSLJZD: Xiangshaliujunzi decoction; NC: Negative control; 5-FU: 5-fluorouracil.