MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 7 The effect of Xiangshaliujunzi decoction on the expression of PANoptosis-related proteins and inflammatory factors was mediated by the miR-200a-3p/ZEB1 axis. Xiangshaliujunzi decoction (XSLJZD) enhanced the expression levels of pyroptosis-related proteins, apoptosis-related proteins, and programmed cell death (PCD)-related proteins. XSLJZD upregulated the secretion of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-18 in BGC-823/5-fluorouracil (5-FU) cells. Transfection of miR-200a-3p inhibitors into BGC-823/5-FU cells partially abolished the effects of XSLJZD on the expression of PANoptosis-related proteins and inflammatory factors. Knocking down ZEB1 expression in BGC-823/5-FU cells transfected with miR-200a-3p inhibitors partially restored the effects of XSLJZD on PANoptosis-related proteins and inflammatory factor expression. A: Expression levels of pyroptosis-related proteins, apoptosis-related proteins, and PCD-related proteins; B: IFN-γ, TNF-α, IL-1β, and IL-18 in cell culture medium detected by ELISA. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs Control. ^dP < 0.05, ^eP < 0.01 vs XSLJZD + in-negative control (NC). ^fP < 0.05 vs XSLJZD + sh-NC. XSLJZD: Xiangshaliujunzi decoction; IFN: Interferon; TNF: Tumor necrosis factor; IL: Interleukin.