MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 6 The effect of Xiangshaliujunzi decoction on cell functions was mediated by the miR-200a-3p/ZEB1 axis. Cell viability and proliferation were significantly decreased, while apoptosis was significantly increased in the Xiangshaliujunzi decoction (XSLJZD)-treated group. Cell apoptosis/necroptosis (YP1 positive) and necroptosis/pyroptosis (PI positive) were also elevated in the XSLJZD-treated group. Transfection of miR-200a-3p inhibitors into BGC-823/5-fluorouracil (5-FU) cells partially reversed the effects of XSLJZD. Knocking down ZEB1 expression in BGC-823/5-FU cells transfected with miR-200a-3p inhibitors partially restored the effects of XSLJZD. A: Cell viability detected by CCK8 assays; B: Cell proliferation detected by colony formation assays; C: Cell apoptosis detected by flow cytometry; D: Cell PANoptosis detected by YO-PRO-1/PI staining. ^bP < 0.01, ^cP < 0.001 vs control. ^dP < 0.05, ^eP < 0.01 vs XSLJZD + in-negative control (NC). ^fP < 0.05 vs XSLJZD + sh-NC. XSLJZD: Xiangshaliujunzi decoction; NC: Negative control.