MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 5 Xiangshaliujunzi decoction inhibited ZEB1 expression by up-regulation of miR-200a-3p. MiR-200a-3p expression was increased in the Xiangshaliujunzi decoction (XSLJZD) group, while ZEB1 expression was significantly decreased. The effect of XSLJZD on miR-200a-3p and ZEB1 expression was dose-dependent. Inhibiting miR-200a-3p expression in the XSLJZD group partially restored ZEB1 expression. A: MiR-200a-3p and ZEB1 mRNA expression; B: ZEB1 protein expression in different treatment groups; C: MiR-200a-3p and ZEB1 mRNA expression in XSLJZD-treated BGC-823/5-fluorouracil cells transfected with miR-200a-3p or ZEB1 knock down. L-XSLJZD: 1.55 g/(kg·day); M-XSLJZD: 3.1 g/(kg·day); H- XSLJZD: 6.2 g/(kg·day). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control. ^dP < 0.05, ^eP < 0.01 vs XSLJZD + in-negative control (NC). ^fP < 0.01 vs XSLJZD + sh-NC. XSLJZD: Xiangshaliujunzi decoction; NC: Negative control.