MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 3 Xiangshaliujunzi decoction promoted the expression of PANoptosis-related proteins. In BGC-823/5-fluorouracil cells treated with Xiangshaliujunzi decoction (XSLJZD), the expression levels of pyroptosis-related proteins (cleaved caspase-1, GSDMD-FL, GSDMD-N, GSDME-FL, GSDME-N), apoptosis-related proteins (cleaved caspase-3, cleaved caspase-7, and cleaved caspase-8), and programmed cell death-related proteins (p-RIPK1, p-MLKL) increased in a dose-dependent manner. A: Western blot bands of PANoptosis proteins; B: Bar chart of relative protein expression levels. L-XSLJZD: 1.55 g/(kg·day); M-XSLJZD: 3.1 g/(kg·day); H-XSLJZD: 6.2 g/(kg·day). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control. XSLJZD: Xiangshaliujunzi decoction.