MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer

Figure 1 Xiangshaliujunzi decoction suppressed cell viability, cell proliferation and promoted apoptosis in a dose-dependent manner. A: Cell viability detected by CCK8 assays; B: Cell proliferation detected by colony formation assays; C: Cell apoptosis detected by flow cytometry. L-Xiangshaliujunzi decoction (XSLJZD): 1.55 g/(kg·day); M-XSLJZD: 3.1 g/(kg·day); H-XSLJZD: 6.2 g/(kg·day). The vertical axis represents optical density at 450 nm from CCK-8 assays (A), normalized to the control at 0 hour (set as 1). ^aP < 0.05, ^bP < 0.01 vs control. One-way ANOVA with Tukey's post-hoc test was used. XSLJZD: Xiangshaliujunzi decoction.